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Ten Common Questions

Q: How do you know where to look for a certain species?
A: You can figure out what species are found where you are going by looking in books or field guides about the type of animal you are interested in. There are no books about the amphibians of Cameroon, so I rely on two different types of information.

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I read the scientific literature so that I know where people have found certain species before. I also use databases of museum collections. A museum’s database will usually list when and where the specimens of a certain species were collected. This is very valuable information to us when we are trying to find more of that species!


Q: How do you find frogs and salamanders?
A: You can look for amphibians in a few ways. If you are looking for salamanders, then there are different ways to look for them depending on where they live. For salamander species that live on land, the easiest way to find them is by looking under or in logs or moss. For species that live in water, you often need to use a net to catch them, although if they live in small streams sometimes you can catch them by hand. The easiest way to catch frogs is to go out in the evening or at night and listen for their calls. The calls will lead you straight to the frog but it is still not always to see the calling frog. Sometimes it is necessary to search in trees, bushes, or grass before you catch the frog. Nets can be used to catch frogs that live in water. We also use nets to catch tadpoles and salamander larvae.

Q: How do you collect and preserve amphibians?
A: When we find and collect amphibian species, we first write down information about the animal. We will write down the time and date that it was found. We will also record basic information about both the weather and the habitat that it was found in. This information is very important for understanding where species live and what they do. We also write down what the animal looks like. This is important because many of the bright colors will fade or disappear after the animal is stored in preservative. An example of these notes from my own field book reads as follows:

“Oku, Northwest Province, Cameroon. September 21, 2004. Approximately 8 PM. Astylosternus ranoides. Found in driveway of Birdlife International Project House during light rain. Driveway is surrounded by farmland and is about 0.5 km from a fast moving stream. The back of the frog is yellow-tan with dark brown markings; the belly is a creamy yellow. The iris of the eye is dark brown.”

In order to preserve the animal, it is important that the animal dies in a humane way. For amphibians, we place them in a jar with a little bit of water and a chemical that makes them go to sleep. It is a painless and they die in their sleep. We then place the animal in an air-tight container with a little bit of formaldehyde. Once the animal is well-preserved (usually 24 hours later), we then store it in alcohol. Museum collections of amphibians and reptiles have thousands of jars filled with alcohol and preserved specimens.

Dave Blackburn
Dave Blackburn working in the Herpetology Collections of Harvard's Museum of Comparative Zoology. Photo K. Blackburn

Q: How do you know if you find a new species?
A: This takes a lot of work! First, it is important to be sure that what you have found is a species that no one else has found before you. To do this, you have to compare the animal that you found to specimens of other species that it is similar to. When describing a new species of frog, we consider all sorts of information. We examine the frogs anatomy (i.e., what it looks like) and this includes the skeleton and even the guts of the frog. We can use DNA to see how different the DNA is from other potentially related species. We can also examine the ecology of the species such as when it breeds, how many eggs it lays, and if the parents provide care for the eggs or tadpoles. Lastly, we often use the calls of the frogs to determine if species are different. Most frog species have calls that are unique to that species. If the anatomy, DNA, ecology, and call all point to the species as being unlike any others that are described, then it is probably a new species!

Q: Do you need permission to collect animals?
A: Yes! It is extremely important to obtain permission to collect animals. You also need permission to take them out the country (export) and to bring them into the country (import). First, you must get permission to collect animals in the country that you are visiting. In Cameroon, I obtain this permission from the Ministry of Environments and Forests. Then you must get permission to export the animals (living or preserved) back to the United States. You must also get permission from the United States to import the specimens. This is a lot of work to do but it is absolutely necessary in order to stop the illegal international trade in animals.

Q: Do you need permission to collect animals in the United States?
A: Yes! When you’re at home in the United States, it is also very important to make sure that it is legal to collect amphibians and reptiles. Even in the United States, there are many species of amphibians and reptiles that are endangered. Just like you need a hunting license to hunt deer, moose, or bears, you need permission to collect amphibians or reptiles in your state. You can find out more about this by contacting your local U.S. Fish and Wildlife Service.

Q: Do you need extra permission to collect endangered animals?
A: Yes! Collecting endangered animals is very different from collecting other animals. Many animals, such as species of apes, large cats, crocodiles, and chameleons, are protected by the Commission on the International Trade of Endangered Species (CITES). Some countries also protect species that are special or meaningful to that particular country. If a species is listed as protected by CITES, then you must get extra permissions from the country where you collect animals. You must also get extra permission from the United States Fish and Wildlife Service when they are imported. All of this helps to ensure that endangered species continue to be protected as much as possible.

Q: How do you get DNA from an animal?
A: Just before we place the animal in preservative, we take a small piece of liver and preserve it in alcohol. When I get back to the laboratory, then I will use this piece of liver to get DNA for molecular research. We use special kits from molecular biology companies to extract the DNA from the tissue. We then use this to get the DNA sequence of particular genes that we are interested in.

Q: What type of school do I have to go to if I want to be a biologist?
A: It is important to complete high school and then to go to college. In college you can major in biology, environmental science, ecology and even geology. This can lead to many different careers. With a biology-related college degree can get jobs as a research assistant in laboratories or even work for the national parks. With a master’s degree or a PhD, you can get jobs as a researcher or professor at a university or a museum

Q: Can I see frogs and salamanders at home?
A: To find frogs and salamanders, you can visit forest preserves or national parks but you can also look in your own backyard or neighborhood park. If you know of nearby ponds or streams, these can be great places to find frogs and salamanders. Toads are common in many neighborhoods. An easy way to find frogs is to go out to ponds, lakes, and forests. Salamanders are often found under logs in the woods.

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All photos by D.C. Blackburn unless otherwise noted
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